Optimization of Extraction and Purification for Jiangtangling Oral Liquid on AHP-entropy Weight Method
Objective To optimize the extraction and purification process of Jiangtangling oral liquid to provide reference for industrial production.Methods Based on AHP-entropy weight method,the four evaluation indexes of total polysaccharide content,total solid content,puerarin and calycosin-7-glucoside were empowered subjectively and objectively.Orthogonal design was used to investigate the material-to-liquid ratio,extraction time and extraction duration in the water extraction process,and the best extraction process was optimized by the comprehensive score of four evaluation indexes.Box-Behnken design was used to investigate the effect of the relative density of the extract,volume fraction of ethanol for precipitation,and precipitation duration in the ethanol precipitation process,and the best purification process was optimized by the comprehensive score of four evaluation indexes.Results The best extraction process of Jiangtangling oral liquid was material-to-liquid ratio of 1∶8,extraction for 1 h with a total of 3 times;the best purification process was to concentrate the extract to relative density of 1.15,add 95％ethanol to 65％alcohol and stand for 12 h.There was no significant difference in the 3 batches of validation tests.Conclusion The preferred extraction and purification process of Jiangtangling oral liquid is stable and reliable,which can provide a theoretical basis for industrial production.
万方数据
维普
