Identify and Trace Contaminated Bacteria in Chinese Patent Drugs by Specific PCR and MLST Methods
Objective To study methods for identification and tracing contaminating bacteria in Chinese patent drugs.Methods In the daily work of microbial enumeration tests,bacteria cultured from 34 batches of Chinese patent drugs were identifed through MALDI-TOF-MS,16S rDNA sequencing and Bacillus specific PCR method.MLST methods were used to type Bacillus subtilis and Bacillus cereus detected both in drugs and lab environment.Results MALID-TOF-MS could identify most bacteria but not Bacillus cereus group and some Bacillus substilis groups which could be discriminated by 16S rDNA sequencing and Bacillus specific PCR method.MLST results showed there were same groups of Bacillus cereus in drugs and environment but different groups of Bacillus subtilis between them.Conclusion Most of the contaminating bacteria in Chinese patent drugs are Bacillus genus.Thus Bacillus specific PCR and MLST methods are quick and efficient ways to identify and trace contaminating bacteria in Chinese patent drugs.
Bacillus specific PCRMLST16S rDNA sequencingMALDI-TOF-MS
NETL
NSTL
万方数据
