Study on the Ameliorating Effect of Ganoderma leucocontextum on Renal Fibrosis via Keap1/Nrf2/HO-1 Signaling Pathway
Objective To explore the ameliorating effect of Ganoderma leucocontextum on renal fibrosis and its potential molecular mechanism by using the mouse unilateral ureteral ligation model.Methods C57BL/6J mice were operated on by UUO to establish renal fibrosis model.After 7 d of administration of 400 and 800 mg/kgGLW,kidney morphological changes were observed and renal function indexes such as serum creatinine and urea nitrogen were detected.The changes of renal structure and collagen deposition were observed by HE and Masson staining.The expressions of Keap1 and Nrf2 proteins were detected by immunohistochemistry.Western Blotting and qPCR were used to detect the expressions of α-SMA,Vimentin,MMP2,COL I,HO-1,Keap1 and Nrf2 in each group.Results In the model group,the kidney swelling was obvious,a large amount of urine was stored,and serum Scr and BUN were significantly increased.Pathology showed atrophy of renal parenchyma,high dilatation of renal pelvis,cell swelling,degeneration and necrosis,and increased collagen deposition.The protein expression and mRNA levels of α-SMA,Vimentin,MMP2,COL I and Keap 1 were significantly increased,while the protein expression and mRNA levels of HO-1 and Nrf2 were significantly decreased.The above changes were significantly reversed after GLW intervention.Conclusion GLW can significantly improve UUO-induced kidney injury and renal fibrosis through Keap1/Nrf2/HO-1 signaling pathway,providing a potential new approach for the treatment of CKD.
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