西南远志水提物缓解体力疲劳和改善睡眠功效研究
Study on the Activity of Water Extract of Polygala crotalarioides Buch.Ham.in Alleviating Physical Fatigue and Improving Sleep
柴琇瑛 1颜宏 1赵道强 1张梦麒 1肖茹月 1王建康 1吴德松1
作者信息
- 1. 云南省药物研究所,云南 昆明 650111;云南省中药和民族药新药创制企业重点实验室,云南 昆明 650111
- 折叠
摘要
目的 对西南远志水提物缓解体力疲劳和改善睡眠功效进行研究,为其进一步开发利用提供数据支持.方法 以小鼠负重游泳时间、小鼠肝糖原、尿素氮和血乳酸水平作为评价指标,研究西南远志水提物的缓解体力疲劳作用;采用延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验和巴比妥钠睡眠潜伏期实验对西南远志改善睡眠功效进行研究.结果 与空白对照组比较,西南远志水提物400 mg/kg剂量可明显提升小鼠负重游泳时间(P＜0.05),200 mg/kg和400 mg/kg剂量可明显提升小鼠肝糖原水平(P＜0.01,P＜0.001),400 mg/kg剂量可明显降低小鼠血清尿素氮水平(P＜0.001),但其各给药剂量对小鼠血乳酸曲线下面积均无明显影响(P＞0.05).与空白对照组比较,西南远志水提物各给药剂量对小鼠睡眠时间和睡眠潜伏期均无明显影响(P＞0.05),但其400 mg/kg剂量可明显提升睡眠小鼠数量(P＜0.05).结论 根据《保健食品功能评价方法》的判定标准,在本研究所设置的剂量范围内,西南远志水提物具有缓解体力疲劳活性,但改善睡眠作用较弱.
Abstract
Objective To study efficacy of water extract of Polygala crotalarioides Buch.Ham.(WEP)in alleviating physical fatigue and improving sleep,in order to provide an experimental basis for its further development.Methods Weight-bearing swimming test,liver glycogen,urea nitrogen and blood lactate levels in mice were used to study the alleviating physical fatigue effect of WEP.The sleep improvement effect of WEP was evaluated by threshold dose pentobarbital sodium sleep test,subthreshold dose pentobarbital sodium sleep test and threshold dose barbital sodium sleep test.Results Compared with the blank control group,the 400 mg/kg of WEP could significantly improve the weight-bearing swimming time in mice(P＜0.05).At the same time,200 mg/kg and 400 mg/kg could significantly increase the level of liver glycogen in mice(P＜0.01,P＜0.001),and 400 mg/kg could obviously reduce the serum urea nitrogen level in mice(P＜0.001).However,each dose of WEP had no significant effect on the area under the blood lactate curve in mice(P＞0.05).Compared with the blank control group,each dose of WEP had no significant effect on sleep time and sleep latency in mice(P＞0.05).On the other hand,400 mg/kg dose of WEP could significantly increase the number of mice sleeping(P＜0.05).Conclusion Within the dose range set by this study,the water extract of Polygala crotalarioides Buch.Ham.has the activity of alleviating physical fatigue,but the improving sleep effect is weak.
关键词
西南远志/体力疲劳/睡眠/保健功能Key words
Polygala crotalarioides Buch.Ham./physical fatigue/sleep/health function引用本文复制引用
出版年
2024
NETL
NSTL
万方数据