Study on Potential Pharmacological Substances and Mechanisms of Qingwen Hufei Granules in Immune Regulation
Objective To explore the potential pharmacological substances and mechanisms of Qingwen Hufei Granules(QWHF)in immune regulation applying network pharmacology and molecular docking technology combined with in vitro and in vivo experiments.Methods Component-target prediction was performed using the BATMAN-TCM database,and targets related immune regulatory were queried through the GeneCards database.The interaction network diagram of intersecting targets was constructed,and core targets were screened.GO and KEGG enrichment analysis were performed using the DVIAD database.The effect of BWJD on RAW264.7 macrophage cell viability was detected using CCK-8 method.Moreover,the release of NO,TNF-α,IL-1β and IL-6 were measured using Griess and ELISA method respectively.The immunosuppressed mouse model was established by cyclophosphamide of intraperitoneal injection at a dose of 80 mg/kg in vivo experiments.Blank control group,model group,QWHF low and high(4.3,8.6 g/kg)dose groups were set up.After intragastric administration for 14 d,the hemogram,spleen and thymus index,immune factor levels in serum and tissue of each group were measured.Results Total of 460 potential targets were identified for the immune regulation in QWHF,and 43 core targets were screened,and 65 potential pharmacological components were identified.The molecular docking results showed that isoliquiritigenin,atractylenolide Ⅲ and liquiritigenin all had good binding ability with receptors such as AKT1,TNF and IL-6.The results indicate that the range of 400 and 1 000 μg/mL QWHF could significantly increase NO secretion,and stimulate release of the inflammatory factor TNF-α,IL-1β and IL-6 in varying degrees.Both low and high dose of QWHF groups could significantly increased the spleen index,the content of white blood cells,neutrophils,and lymphocytes in immunocompromised mice,meanwhile TNF-α and IL-1 β in serum,spleen and thymic,as well as IgG,IgM in serum and SOD secretion in thymic also increased significantly.Conclusion The study preliminarily revealed the material basis and mechanism of immune regulatory efficacy of QWHF,which provide a reference for its clinical research and further development.
NETL
NSTL
万方数据
