Aiming at the problems of high stroke rate,short stroke and crankshaft failure of traditional mechanical fracturing pump,a new type of hydraulic fracturing pump driven by multi-cylinder combined flow was proposed and the mechanism of its flow pulsation was studied.Firstly,the working principle of multi-cylinder combined flow fracturing pump was described in detail.And the mathematical model of the multi-cylinder combined flow fracturing pump was established and analyzed by using the theory of hydraulic fluid mechanics.Then,the simulation model of the multi-cylinder combined flow fracturing pump was built by AMESim.In addition,a reasonable simulation parameter setting was selected to carry out simulation research under variable load pressure.Finally,the piston-plunger movement characteristics,pump chamber flow characteristics,output flow and pressure pulsation characteristics in the hydraulic power transmission chain were studied,and the simulation results were analyzed.The research results show that under the condition of 25 MPa,the output pressure and flow pulsation rate of multi-cylinder combined flow fracturing pump are respectively36.8%and20.61%,and the stroke times are only42 times/min,which accord with the actual working condition.The main reasons for the output flow pulsation of the multi-cylinder combined flow fracturing pump are the frequent reversal of each cylinder,the switch of pumping stroke and suction stroke,which lead to the deceleration stop motion and acceleration start motion of the piston and plunger.