DIVERSITY ANALYSIS OF FUNGI ISOLATED FROM NY-(A)LESUND,SVALBARD, AND SCREENING FOR BIOTRANSFORMATION OF GENTIOPICROSIDE
Sixty-five strains of fungi were isolated from soil and coastal marine sediment samples in Ny-Alesund,on the Arctic island of Spitzbergen,Svalbard.Phylogenetic diversity analysis was conducted using 18S rDNA sequencing.Classification based on 18S rDNA showed that the 65 isolated fungal strains belonged to 25 genera.Among them,20 strains were Penicillium strains,six were Nectria and another six were Articulospora strains.Screening of the β-glucosidase activity of these strains was carried out,and 28 positive strains were obtained.Among them,nine strains showing higher β-glucosidase activity were further analyzed to detect biotransformation of gentiopicroside.Following TLC and HPLC analysis of the transformed crude extracts,the strain C-5 showed a special transformation role with the potential for new transformed products.According to the morphological characteristics and nucleic acid sequence,it was identified as most likely belonging to the genus Penicillium,and was provisionally named Penicillium sp.C-5.
Arcticfungidiversityβ-glucosidasebiotransformation of gentiopicroside
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