Preliminary Study on Leaf Tissue Culture of Endangered Plant Sinomanglietia glauca
Sinomanglietia glauca is a precious and endangered tree species in Magnoliaceae family,with important potential application value.In this study,the optimal disinfection time,optimal hormone concentration ratio and optimal culture medium to induce callus were explored with leaves of Sinomanglietia glauca through single factor experiment.The results showed that:(1)8 minutes was the optimal disinfection time,with 0.1% mercuric chloride solution(HgCl2)as disinfectant;(2)MS medium was the optimal basic medium for tissue culture of leaves of Sinomanglietia glauca;(3)The optimal hormone ratio for inducing callus formation in leaves was MS+2.0 mg/L 6-BA+0.2 mg/L IBA.These results are a summary of experience in tissue culture techniques of Sinomanglietia glauca,and have practical significance for its protection and utilization.
Sinomanglietia glaucadisinfection durationhormone concentration ratioculture medium
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