首页|Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches

Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches

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The bacterial community of a bulking sludge from a municipal wastewater treatment plant with anoxic-anaerobic-oxic process was investigated by combination of cultivation and 16S rRNA gene clone library analysis for understanding the causes of bulking.A total of 28 species were obtained from 63 isolates collected from six culture media.The most cultivable species belonged to γ-Proteobacteria including Klebsiella sp.,Pseudomonas sp.,Aeromonas sp.and Acinetobacter sp.Further analysis of these strains by repetitive sequence based on polymerase chain reaction (rep-PCR) technology showed that rep-PCR yielded discriminatory banding patterns within the same genus using REP and BOX primer sets.While the culture-independent assessment revealed that β-Proteobacteria was the dominant group in the bulking sample.Sequence analysis revealed that the highest proportion (14.7%) of operational taxonomic units was 98% similar to Candidatus Accumulibacter phosphatis,which is used to remove phosphorous from wastewater.Our results indicated that combining different approaches can produce complementary information,thus generate a more accurate view of microbial community in bulking sludge.

bulking sludgecultivable bacteriarep-PCR16S rRNA gene clone library

Decai Jin、Ping Wang、Zhihui Bai、Xinxin Wang、Hong Peng、Rong Qi、Zhisheng Yu、Guoqiang Zhuang

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Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Graduate University of Chinese Academy of Sciences, Beijing 100049, China

School of Chemical Engineering, The University of Queensland, Brisbane, Queensland 4072, Australia

This work was supported by the "Knowledge Innovation" Program of Chinese Academy of SciencesThis work was supported by the "Knowledge Innovation" Program of Chinese Academy of SciencesMinistry of Science and Technology,China

KZCX2-YW-JC407-3KSCX2-YW-G-054-22006DFA91870

2011

10.1016/S1001-0742(10)60621-3

环境科学学报(英文版)
中科院生态环境研究中心

环境科学学报(英文版)

CSTPCDCSCDSCI
影响因子：0.862
ISSN：1001-0742
年,卷(期)：2011.23(11)
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