A screen-printed, amperometric biosensor for the determination of organophosphorus pesticides in water samples

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外文摘要：An amperometric biosensor based on screen-printed electrodes (SPEs) was developed for the determination of organophosphorus pesticides in water samples.The extent of acetylcholinesterase (AChE) deactivation was determined and quantified for pesticideconcentrations in water samples.An enzyme immobilization adsorption procedure and polyacrylamide gel matrix polymerization were used for fabrication of the biosensor,with minimal losses in enzyme activity.The optimal conditions for enzyme catalytic reaction on the SPEs surfaces were acetylthiocholine chloride (ATChC(1)) concentration of 5 mmol/L,pH 7 and reaction time of 4 min.The detectionlimits for three organophosphorus pesticides (dichlorvos,monocrotophs and parathion) were in the range of 4 to 7 μg/L when an AChE amount of 0.1 U was used for immobilization.

外文关键词：

organophosphorus pesticidesacetylcholinesteraseenzyme immobilizationscreen-printed electrodesbiosensor

作者：

Junfeng Dou、Fuqiang Fan、Aizhong Ding、Lirong Cheng、Raju Sekar、Hongting Wang、Shuairan Li

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作者单位：

College of Water Sciences, Beijing Normal University, Beijing 100875, China

Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou 215123, China

基金：

This work was supported by the National Natural Science Foundation of ChinaThis work was supported by the National Natural Science Foundation of ChinaThis work was supported by the National Natural Science Foundation of China

项目编号：

510080264087307641011130204

出版年：

2012

DOI：

10.1016/S1001-0742(11)60864-4

环境科学学报(英文版)

中科院生态环境研究中心

环境科学学报(英文版)

CSTPCDCSCDSCI

影响因子：0.862

ISSN：1001-0742

年,卷(期)：2012.24(5)

参考文献量31