首页|Degradation of gaseous hydrocarbons in aerated stirred bioreactors inoculated with Rhodococcus erythropolis:Effect of the carbon source and SIFT-MS method development

Degradation of gaseous hydrocarbons in aerated stirred bioreactors inoculated with Rhodococcus erythropolis:Effect of the carbon source and SIFT-MS method development

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
Degradation of gaseous hydrocarbons in aerated stirred bioreactors inoculated with Rhodococcus erythropolis:Effect of the carbon source and SIFT-MS method development
The study of microbial hydrocarbons removal is of great importance for the development of future bioremediation strategies.In this study,we evaluated the removal of a gaseous mix-ture containing toluene,m-xylene,ethylbenzene,cyclohexane,butane,pentane,hexane and heptane in aerated stirred bioreactors inoculated with Rhodococcus erythropolis and operated under non-sterile conditions.For the real-time measurement of hydrocarbons,a novel sys-tematic approach was implemented using Selected-Ion Flow Tube Mass Spectrometry(SIFT-MS).The effect of the carbon source(～9.5 ppmv)on(i)the bioreactors'performance(BR1:dosed with only cyclohexane as a single hydrocarbon versus BR2:dosed with a mixture of the 8 hydrocarbons)and(ii)the evolution of microbial communities over time were investi-gated.The results showed that cyclohexane reached a maximum removal efficiency(RE)of 53％±4％in BR1.In BR2,almost complete removal of toluene,m-xylene and ethylbenzene,being the most water-soluble and easy-to-degrade carbon sources,was observed.REs below 32％were obtained for the remaining compounds.By exposing the microbial consortium to only the five most recalcitrant hydrocarbons,REs between 45％±5％and 98％±1％were reached.In addition,we observed that airborne microorganisms populated the bioreactors and that the type of carbon source influenced the microbial communities developed.The abundance of species belonging to the genus Rhodococcus was below 10％in all bioreactors at the end of the experiments.This work provides fundamental insights to understand the complex behavior of gaseous hydrocarbon mixtures in bioreactors,along with a systematic approach for the development of SIFT-MS methods.

HydrocarbonsSIFT-MSRhodococcus erythropolisBiodegradationBioreactors

Paula Alejandra Lamprea Pineda、Kristof Demeestere、Allan Augusto Alvarado-Alvarado、Frank Devlieghere、Nico Boon、Herman Van Langenhove、Christophe Walgraeue

展开 >

Research group EnVOC,Department of Green Chemistry and Technology,Faculty of Bioscience Engineering,Ghent University,Coupure Links 653,9000,Ghent Belgium

Research group FMFP,Department of Food Technology,Safety and Health,Faculty of Bioscience Engineering,Ghent University,Coupure Links 653,9000,Ghent Belgium

Center for Microbial Ecology and Technology-CMET,Department of Biotechnology,Faculty of Bioscience Engineering,Ghent University,Coupure Links 653,9000,Ghent Belgium

Hydrocarbons SIFT-MS Rhodococcus erythropolis Biodegradation Bioreactors

2025

10.1016/j.jes.2023.10.020

环境科学学报(英文版)
中科院生态环境研究中心

环境科学学报(英文版)

影响因子：0.862
ISSN：1001-0742
年,卷(期)：2025.147(1)