隐丹参酮对铜绿假单胞菌的生长及生物膜形成的抑制作用

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国家科技期刊平台
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中文摘要：为研究隐丹参酮(cryptotanshinone)对铜绿假单胞菌(Pseudomonas aeruginosa)的生长和生物膜形成的影响及其机制,采用微量稀释法测定隐丹参酮对铜绿假单胞菌的最小抑菌质量浓度(MIC),利用结晶紫染色法、倒置显微镜和激光扫描共聚焦显微镜观察隐丹参酮对铜绿假单胞菌生物膜形成的影响,再采用实时荧光定量聚合酶链反应(RT-qPCR)检测隐丹参酮对铜绿假单胞菌生物膜形成相关基因LasI、LasR、RhlI和RhlR的表达的影响.隐丹参酮对铜绿假单胞菌的MIC为800.0 μg/mL;结晶紫染色后,隐丹参酮的质量浓度为800.0 μg/mL时抑制菌膜效果最佳,且呈剂量依赖性(P<0.01);利用激光共聚焦显微镜观察隐丹参酮对生物膜的形成和分布,200.0 μg/mL时抑菌效果显著提升.在200.0～800.0 μg/mL的质量浓度范围内,800.0 μg/mL的隐丹参酮对LasI、LasR、RhlI和RhlR基因的表达具有抑制作用(P<0.01).隐丹参酮抑制铜绿假单胞菌生物膜形成机制可能是通过下调LasI等基因的表达.本研究表明,隐丹参酮可以作为群体感应抑制剂干预铜绿假单胞菌的耐药,为临床多重耐药铜绿假单胞菌(MDR-PA)的治疗提供参考.

外文标题：Inhibitory Effect of Cryptotanshinone on Biofilm Formation of Pseudomonas aeruginosa

外文摘要：To investigate the effects of cryptotanshinone on the growth and biofilm formation of Pseudomonas aeruginosa and its mechanism,the minimum inhibitory concentration(MIC)of cryptotanshinone on P.aeruginosa was determined by mi-crodilution,and the effect of cryptotanshinone on P.aeruginosa biofilm formation was observed by crystal violet staining,inverted microscope and laser scanning confocal microscope,and the effect of cryptotanshinone on P.aeruginosa biofilm for-mation was measured by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).The effect of cryptotanshi-none on the expression of genes related to P.aeruginosa biofilm formation was detected by RT-qPCR.The MIC of cryptotan-shinone on P.aeruginosa was 800.0 μg/mL;after crystal violet staining,the best inhibition of biofilm was observed at the mass concentration of cryptotanshinone of 800.0 μg/mL in a dose-dependent manner(P<0.01).The formation and distribution of biofilm by cryptotanshinone were observed by confocal laser microscopy,and the inhibition effect was significantly enhanced at 200.0 μg/mL.In the range of 200.0～800.0 μg/mL mass concentration,cryptotanshinone at 800.0 μg/mL mass concentra-tion inhibited the expression of LasI,LasR,RhlI and RhlR genes(P<0.01).The mechanism of cryptotanshinone inhibition of P.aeruginosa biofilm formation may be through the down-regulation of LasI and other genes expression.The present study suggests that cryptotanshinone can be used as a group-sensing inhibitor to intervene in the drug resistance of P.aeruginosa,which can provide a reference for the treatment of multi-drug-resistant P.aeruginosa(MDR-PA)in the clinic.

外文关键词：

Pseudomonas aeruginosabiofilmcryptotanshinonequorum sensingdrug resistance

作者：

马书恒、缪林坪、陈荻秋、韩丽、张富凯、贺气志

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作者单位：

长沙医学院第一临床学院,长沙 410219

长沙医学院基础医学院,长沙 410219

新型药物制剂研发湖南省重点实验室,长沙 410219

关键词：

铜绿假单胞菌生物膜隐丹参酮群感系统耐药性

基金：

国家自然科学基金青年项目湖南省教育厅科学研究项目湖南省大学生创新创业训练项目湖南省大学生创新创业训练计划重点支持领域项目长沙医学院大学生创新创业训练项目

项目编号：

82100040湘教通[2022]323号-22B0899湘教通[2021]197号-3905湘教通[2023]237号-38长医教[2023]51号-108

出版年：

2024

DOI：

10.3969/j.issn.1007-7146.2024.01.011

激光生物学报

中国遗传学会

激光生物学报

CSTPCD

影响因子：0.378

ISSN：1007-7146

年,卷(期)：2024.33(1)

参考文献量25