Screening and Identification of Linear Epitopes on the VP1 Protein of Coxsackievirus A10
Coxsackievirus A10(CV-A10)is one of the most common pathogens causing hand,foot and mouth disease(HFMD).The 39 candidate epitope peptides covering the amino acid sequence of the VP1 region of CV-A10 were screened by using the overlapping peptide method and verified by indirect enzyme linked immunosorbent assay(ELISA)and microneutral-ization inhibition assays.It has been found that candidate epitope P6(amino acid 39～53 in VP1 region of CV-A10)showed high reactivity with CV-A10 virus serum,and still showed neutralizing inhibition effect at 3.91 μg/mL dilution mass concentration,which was a potential neutralizing epitope.P6 antiserum was prepared by immunizing mice with P6 peptide,and microneutral-ization test was used to verify its protective effect.The results showed a 1:8.97 geometric mean neutralizing titer for P6 antise-rum,which identified P6 as the neutralizing epitope of CV-A10.In order to understand the conservativeness of P6 sequence in CV-A10,the amino acid sequence of the P6 was compared with the representative strains of CV-A10 genotype,and the results showed that P6 was highly conserved in CV-A10.It indicated that P6 is a conserved linear neutralization epitope of CV-A10,which has the potential to resist the infection of various genotypes of CV-A10,and can be used as a target for the development of CV-A10 epitope vaccine.This study aimed to screen and identify linearly neutralizing epitopes on the CV-A10 VP1 protein and to lay a foundation for the development of CV-A10 epitope vaccine and HFMD prevention.
coxsackievirus A10VP1 proteinlinear neutral epitopevaccinehand,foot and mouth disease
国家科技期刊平台
NSTL
万方数据
维普
