Effects of Ginkgolide B on Proliferation and Apoptosis of Esophageal Cancer Cells by Regulating JAK2/STAT3 Signaling Pathway
To investigate the effects of ginkgolide B on the proliferation and apoptosis of esophageal cancer cells and the related mechanisms.Human esophageal cancer OE19 cells were cultured in vitro.They were divided into control group(no intervention),low/medium/high dose test group(adding 6.25,12.50 and 25.00 μmol/L ginkgolide B,respectively),ginkgolide B group(12.50 μmol/L ginkgolide B),positive drug group(4 mg/L cisplatin)and inhibitor group[12.50 μmol/L ginkgolide B+10.00 μmol/L ja-nus kinase 2/transcriptional activator 3(JAK2/STAT3)pathway inhibitor AG490]and activator group(12.50 μmol/L ginkgolide B+0.50 μmol/L JAK2/STAT3 pathway activator Colivelin),24 h after intervention,cell count kit 8(CCK-8),5-acetyl-2'deoxy-uridine(EdU)method,Hoechst 33258 staining,real-time fluorescence quantitative PCR(RT-qPCR)and protein immunoblot(WB)were used to detect cell viability,proliferation rate,apoptosis rate and expression levels of related factors.The results show that:compared with the control group,the cell viability of the medium/high dose test group and positive drug group was decreased(P<0.05),therefore,in this study,12.50 μmol/L ginkgolide B group with significant difference and lower concentration was se-lected as ginkgolide B group for follow-up tests.Compared with the control group,the cell proliferation rate,Cyclin D1 mRNA and protein expression levels,p-JAK2 and p-STAT3 protein expression levels of ginkgolide B group and positive drug group were decreased,while the apoptosis rate and Caspase-3 mRNA and protein expression levels were increased(P<0.05).Compared with the ginkgolide B group,the changes of all indexes in inhibitor group were further enhanced(P<0.05).while those in activator group were significantly reversed(P<0.05).Ginkgolide B can inhibit the proliferation of OE19 cells and promote apoptosis of esophageal cancer cells by down-regulating JAK2/STAT3 signaling pathway.This study revealed a new anticancer mechanism of ginkgolide B and provided a theoretical basis for the study of esophageal cancer.
国家科技期刊平台
NSTL
万方数据
维普
