冬凌草甲素对人软骨肉瘤SW-1353细胞迁移和侵袭能力的影响
Effects of Oridonin on Migration and Invasion of Human Chondrosarcoma SW-1353 Cells
许超 1李震 1孟迪 1李鋆1
作者信息
- 1. 秦皇岛市北戴河医院骨二科,秦皇岛 066100
- 折叠
摘要
为探究冬凌草甲素对人软骨肉瘤细胞迁移和侵袭能力的影响,体外培养人软骨肉瘤SW-1353细胞,将试验分别设为对照组(不做干预),1.25、2.50、5.00 μmol/L冬凌草甲素组(1.25、2.50、5.00 μmol/L冬凌草甲素)和阳性药物组(6 μmol/L阿霉素),检测人软骨肉瘤SW-1353细胞的活力、迁移率、侵袭能力、上皮-间质转化(EMT)蛋白表达量.结果显示:与对照组比较,5.00 μmol/L冬凌草甲素组和阳性药物组人软骨肉瘤SW-1353细胞活力降低(P<0.05);1.25、2.50、5.00 μmol/L冬凌草甲素组和阳性药物组迁移率及侵袭能力降低,且浓度越高变化越显著(P<0.05).冬凌草甲素能够通过升高E-钙黏蛋白(E-cadherin)的表达、降低波形蛋白(vimentin)和N-钙黏蛋白(N-cadherin)的表达量进而抑制EMT进程,且浓度越高变化越显著(P<0.05).冬凌草甲素可显著抑制人软骨肉瘤SW-1353细胞的迁移和侵袭,或是通过介导EMT实现的,这为人软骨肉瘤细胞的生物学研究提供了理论基础.
Abstract
To investigate the effects of oridonin on migration and invasion of human chondrosarcoma cells.In vitro experi-ments,human chondrosarcoma SW-1353 cells were divided into control group being without intervention,1.25,2.50,5.00 μmol/L oridonin groups(1.25,2.50,5.00 μmol/L oridonin)and positive drug group(6 μmol/L adriamycin).Human chondro-sarcoma SW-1353 cells were tested for viability,mobility,invasion ability and epithelial-mesenchymal transition(EMT)expres-sion.The results show that:compared with the control group,SW-1353 cell viability in the 5.00 μmol/L oridonin group and positive drug group significantly decreased(P<0.05).1.25,2.50,5.00 μmol/L oridonin groups and positive drug obviously in-hibted cell migration and invasion in a concentration-dependent manner(P<0.05).The expression of E-cadherin decreased the expression of vimentin and N-cadherin and inhibited EMT process by increasing the expression of E-cadherin protein,and the higher the concentration,the more significant the change was(P<0.05).Lamonidin can significantly inhibit the migration and invasion of human chondrosarcoma SW-1353 cells,or through mediating EMT,which provides a theoretical basis for the bio-logical study of human chondrosarcoma cells.
关键词
冬凌草甲素/软骨肉瘤/SW-1353/迁移/侵袭Key words
oridonin/chondrosarcoma/SW-1353/migration/invasion引用本文复制引用
基金项目
秦皇岛市科学技术研究与发展计划(2023)(202301A266)
出版年
2024
国家科技期刊平台
NSTL
万方数据