The Two-component System YvrG/YvrH Regulates the Biosynthesis of Edeine
Edeine,synthesized by Brevibacillus brevis X23,is a linear peptide antibiotic not encoded by ribosomal RNA,re-nowned for its extensive range of antibacterial effectiveness.Transcriptome sequencing revealed an inverse expression pattern between the two-component regulatory factors yvrG/yvrH and the edeine biosynthesis gene cluster(ede BGC),suggesting the involvement of YvrH/YvrG in the transcriptional regulation of ede BGC.This study utilized Red/ET homologous recombination technology to construct X23 mutant strains that yvrG/yvrH lacks,complemented strains,and overexpressing strains,and inves-tigated the impact of YvrG/YvrH on edeine biosynthesis through phenotype analysis and quantitative real-time PCR(RT-qPCR)techniques.The results revealed that knocking out yvrG/yvrH significantly reduced the antibacterial activity of the strains,the expression of ede BGC decreased,the synthesis of edeine was inhibited,and the yield of edeine was reduced by 46.75%.In summary,YvrG/YvrH act as positive regulatory factors in the edeine biosynthesis.This study provides gene regulatory elements for the metabolic engineering of edeine.
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