Effect of GluA on Growth Development and Butenyl-spinosyn Biosynthesis in Saccharopolyspora pogona
Glutamate transporter GluA,is a class of ATP-binding proteins that can affect intracellular carbon metabolism,ni-trogen metabolism and secondary metabolism by regulating the active transport of glutamate,belonging to the ABC transport system.To study the effects of GluA on the strain growth development and butenyl-spinosyn biosynthesis in Saccharopolyspo-ra pogona,gluA-1 overexpression and deletion strains were constructed by genetic engineering technology.Phenotypic analy-sis showed that the butenyl-spinosyn production increased by 4.2 times,the bacterial growth logarithmic phase was prolonged,the maximum bacterial density and acetyl-CoA content significantly increased,whereas the mycelium morphology was not sig-nificantly changed in overexpressed strain S.pogona::gluA-1.The butenyl-spinosyn production decreased by 37%,the strain growth was inhibited obviously,the acetyl-CoA content significantly decreased,and the mycelium was shortened and thickened,branching was reduced in deletion strain S.pogona-ΔgluA-1.These results indicated that GluA could promote the growth de-velopment and the butenyl-spinosyn biosynthesis in S.pogona,which laid an important foundation for the study of GluA's role in the secondary metabolism of Streptomyces.
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