THE ESTABLISHMENT OF THE REGENERATION SYSTEM OF PUERARIA THOMSONII BENTH
In order to establish an efficient tissue culture regeneration system of Pueraria thomsonii Benth,the effects of different disinfection methods,basic medium,sucrose concentration and explants on the primary culture,secondary culture and rooting culture of P.thomsonii were studied.The results showed that the survival rate of explants was the highest when disinfected with 75%alcohol for 30 s + 0.1%mercury + Tween-80 for 8 min,reaching 83.33%.The best part of the explants was the stem segment with buds,the average plant height could reach 2.13 cm,and the reproduction coefficient was 2.MS was the best basic medium with a reproductive coefficient of 1.26,sucrose concentration was the best,the average plant height was 1.69 cm,the reproductive coefficient was 1.55,the optimal primary medium was MS + agar 5.5 g/L+ sucrose 30 g/L + 6-BA0.2 mg/L + IBA0.6 mg/L,the average plant height was 3.13 cm,the reproductive coefficient was 3.17,and the optimal submedium was MS + agar 5.5g/L+ sucrose 30 g/L + 6-BA0.6 mg/L+ IBA0.8 mg/L+ KT0.2 mg/L,the average plant height was 1.86 cm,the reproduction coefficient was 3.44,and the optimal rooting medium was MS + agar 5.5 g/L + sucrose 30g/L + IBA1.0 mg/L,the rooting rate was 100%,and the average number of taproots was 7.1.The regeneration system of P.thomsonii was established by optimizing tissue culture conditions.
Pueraria thomsonii benthplant tissue cultureregeneration system
国家科技期刊平台
NSTL
万方数据
维普
