DETERMINATION OF POLYPEPTIDE CONTENT IN PERIPLANETA AMERICANA BY FLUORESCENCE ANALYSIS
Fluorescence analysis is an economical and rapid analytical method,which has the advantages of high sensitivity,good selectivity and simple operation.In view of the fluorescent properties of Periplaneta americano polypeptide(DPSFNSWG-NH2),a new method for fluorescence detection of P.americano polypeptide(DPSFNSWG-NH2)was developed.The experimental results showed that under the best conditions,there was a good linear relationship at the concentration of 0.12～31.25 pg/mL.The linear regression equation was y=10.698x+14.625(R2=0.9942),the detection limit was 0.09 pg/mL.The new method was applied to the determination of DPSFNSWG-NH2 in P.americano.The recovery rate was 85.50％～89.47％.This method is simple,rapid and accurate,and has a good application prospect for the determination of DPSFNSWG-NH2 polypeptide contents in P.americano.
万方数据
维普
