METABOLOMIC STUDY ON EFFECTS OF PORTULACA OLERACEA L.POLYSACCHARIDES ON FECAL METABOLITES IN AGED RATS
To investigate the effects of Portulaca oleracea polysaccharides(POP)on fecal metabolites in aged rats,and provide a theoretical basis for its development and utilization of functional foods for the elderly,twenty aged Sprague-Dawley(SD)rats(21 months old)were randomly divided into two groups with ten rats in each group.Control group(Con)rats were fed with laboratory maintenance pellets,and supplementary feeding group(POP)rats were fed with mixed laboratory maintenance pellets(plus 1.0％POP)with free feeding and drinking for 15 weeks consecutively.Then,the automatic biochemical instrument and hematoxylin-eosin staining respectively were used to detect the biochemical indicators in serum,and observe the changes of the tissue morphology of the colon.The levels of short chain fatty acids(SCFAs)in colon contents were analyzed using GC-MS.LC-MS technology was used to detect the feces samples,identify the differential metabolites,and analyze the metabolic pathways.The results showed as follows:(1)Supplements of P.oleracea polysaccharides can significantly reduce total cholesterol,triglycerides,low-density lipoprotein cholesterol,and direct bilirubin levels in the serum of aged rats.(2)Regular morphology and clearer and more intact structures of the colons,and the significantly increased levels of propionic acid and Hexanoic acid in the colon contents were observed in the aged rats treated with POP.(3)A total of 10 differential metabolites with high separation contribution were screened in feces of the two groups,and significant differences in metabolic pathways were obtained,including linoleic acid metabolism,arachidonic acid metabolism,primary bile acid biosynthesis,serotonergic synapse,pyrimidine metabolism,histidine metabolism,and valise,leucine and isoleucine biosynthesis.(4)Triglycerides,low-density lipoprotein cholesterol,and creatinine levels in the serum are associated with 11-hydroperoxy-12,13-epoxy-9-octadecenoic acid,methenamine,taurochenodeoxycholic acid,and 7alpha-hydroxy-3-oxo-4-cholestenoate,respectively.The results indicate that P.oleracea polysaccharides may alleviate the blood lipid levels and direct bilirubin levels in aged rats by affecting metabolites in feces,thereby benefiting the health of the elderly body.
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