IN VITRO ANTIOXIDANT ACTIVITY AND IMMUNE PRE-PROTECTION OF FLAVONOIDS EXTRACT FROM RUBUS VINIFERA
Flavonoids of Rubus vinifera were extracted by different extraction methods,and the contents of flavonoids and its antioxidant activity were determined to optimize the extraction method.The immunosuppressive model was constructed with cyclophosphamide(CTX).24 mice were divided into blank group,cyclophosphamide group,low dose group and high dose group.At the beginning of the experiment,100 and 200 mg/mL of the low and high dose groups of R.vinifera extract were orally administered daily from 1 to 24 days,and the blank group and the cyclophosphamide group were given distilled water.From 19 to 24 days,80 mg/kg CTX was injected intraperitoneally into the model group,low-dose and high-dose extracts groups,respectively,and the same volume of normal saline was injected into the blank group.During the experiment,the body weight and feed intake of the mice were continuously observed.After 5 days of continuous injection of CTX,the spleen,liver weight and spleen tissue morphology were measured,and the contents of malondialdehyde(MDA)and superoxide dismutase(SOD)in serum were detected.The results showed that the ethanol extract had the highest flavonoid contents,and the methanol extract had the best antioxidant activity in vitro.Compared with the cyclophosphamide group,the extract of R.vinifera could reduce the atrophy of spleen and liver in mice,repair the morphological structure damage of spleen tissue in mice,reduce the contents of MDA in serum,increase the activity of SOD,and have a certain protective effect on CTX-induced immunosuppressive mice.
万方数据
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