亚20nm荧光超分辨显微技术研究进展(特邀)
Advances in Sub-20-nm Fluorescence Super-Resolution Microscopy(Invited)
何辰颖 1詹政以 1李传康 1黄宇然 1匡翠方 2刘旭1
作者信息
- 1. 浙江大学光电科学与工程学院,极端光学技术与仪器全国重点实验室,浙江 杭州 310027
- 2. 浙江大学光电科学与工程学院,极端光学技术与仪器全国重点实验室,浙江 杭州 310027;浙江大学杭州国际科创中心,浙江 杭州 311215
- 折叠
摘要
荧光超分辨显微技术自20世纪90年代诞生以来,经历了多代创新与发展,其空间分辨率已经远超衍射极限,横向分辨率能够达20 nm以下,可以实现分子尺度的生物成像与动态追踪.新一代超高分辨率显微技术的产生得益于传统超分辨技术的深度发展和结合创新.详细介绍横向分辨率在亚20 nm尺度的新一代荧光超分辨显微技术,并阐述其与传统超分辨原理的联系与区别.此外,针对分辨率的限制因素,就光学系统、扫描策略和样品制备等方面进行探讨,并展望高分辨率荧光显微技术在生物医学领域中的应用前景和发展方向.
Abstract
Fluorescence super-resolution microscopy has undergone several rounds of innovation and development since the 1990s.The spatial resolution of fluorescence super-resolution microscopy exceeds the diffraction limit by a wide margin,achieving a lateral resolution below to～20 nm and enabling imaging and tracking at the molecular level.The latest generation of ultrahigh-resolution microscopy is the product of extensive development and innovative combination of conventional super-resolution techniques.This review aimed to introduce the new generation of fluorescence super-resolution microscopy at a sub-20-nm scale lateral resolution,and we also elucidated their similarities and differences compared with the conventional super-resolution techniques.Additionally,this review explored how emerging technologies optimize optical systems,scanning strategies,and sample preparation to improve resolution.Finally,we discussed the potential applications and development of high-resolution fluorescence microscopy in biomedical research.
关键词
荧光显微/超分辨成像/调制照明/单分子定位/扫描策略Key words
fluorescence microscopy/super-resolution imaging/modulated illumination/single-molecule localization/scanning strategy引用本文复制引用
基金项目
国家自然科学基金(62125504)
国家自然科学基金(62205288)
中央高校基本科研业务费专项(226-2022-00201)
浙江省"万人计划"科技创新领军人才项目(2020R52001)
出版年
2024
NETL
NSTL
万方数据