Advances in Deep-Tissue Light-Sheet Fluorescence Microscopic Imaging(Invited)
As biomedical research delves deeper into the intricacies of tissue structure and function,the demand for high-resolution,high-signal-to-noise-ratio deep-tissue imaging technologies has become critical.Traditional microscopy,limited to two-dimensional,transparent thin biological samples,falls short in satisfying the current requirements for three-dimensional deep-tissue imaging in biomedical sciences.In contrast,light-sheet fluorescence microscopy distinguishes itself through its attributes—low photodamage,rapid acquisition,extensive field of view,and volumetric imaging—establishing it as a cornerstone among biologists.Despite these advantages,the intrinsic high-scattering characteristics of biological tissues pose formidable challenges to achieving deep imaging.This review focuses on recent progress in light-sheet fluorescence microscopy for deep tissue imaging,emphasizing strategies that overcome challenges associated with high-scattering samples,so as to provide valuable insights to researchers in related fields,assisting them in developing a preliminary comprehension of the latest innovations and future possibilities inherent in this cutting-edge technology.Firstly,the basic principles of light-sheet fluorescence microscopy and the causes and effects of high-scattering absorption characteristics are explained.Subsequently,recent progress in enhancing tissue penetration depth and addressing issues such as light scattering and absorption is further examined.Finally,the development prospects and potential applications of light-sheet fluorescence microscopy imaging technology with high penetration depth and anti-scattering ability are discussed.
NETL
NSTL
万方数据
