Light sheet microscope is an important tool for three-dimensional imaging owing to its powerful optical section capability,fast imaging speed,and low phototoxicity.This microscope typically uses two objectives that are placed perpendicular to each other for illumination and imaging;however,this structure imposes spatial constraints and prohibits the use of an imaging objective with high numerical aperture.These limitations were resolved using single-objective light sheet microscopy techniques,such as oblique plane illumination and micromirror microdevice reflection,demonstrating potential for high-resolution and volumetrically high-speed imaging.Furthermore,these techniques can be combined with various other techniques,such as super-resolution microscopy,which has made great progress in recent years.This review article introduces the principles,key performance enhancements,and biomedical applications of single-objective light sheet microscopy.
维普
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