摘要
荧光干扰问题是拉曼光谱分析中的一大挑战,强荧光会严重影响拉曼光谱信号的检测.介绍了一种去除荧光干扰的移频激发差分拉曼光谱技术,同时介绍了几种差分拉曼光谱复原方法(积分法、曲线拟合法、解卷积法等),并对其原理、实现方式、性能特点进行了讨论.此外,还具体分析了差分拉曼光谱技术在食品、医学、农业等领域的应用情况,可知移频激发差分拉曼光谱具有广泛的应用前景.
Abstract
Fluorescence interference is a major challenge in Raman spectroscopy,as strong fluorescence will seriously affect the detection of Raman spectroscopy signals.This paper introduces several differential Raman spectrum restoration methods(integration method,curve fitting method,deconvolution method,etc.)of shifted excitation Raman difference spectroscopy for defluorescence technology,and discusses its principle,implementation,and performance characteristics.In addition,the application of differential Raman spectroscopy in food,medicine,agriculture and other fields is analyzed in detail,which shows that shifted excitation Raman difference spectroscopy has broad application prospects.
基金项目
国家自然科学基金(62003047)
北京市市属高校优秀青年人才培育计划(2022)(BPHR202203230)
NETL
NSTL
万方数据