荧光共振能量转移光微流激光偏振特性分析
Polarization Characterization of Fluorescence Resonance Energy Transfer Optofluidic Lasers
赵佳奇 1张婷婷 1张天俊 1王文杰 1刘绍鼎1
作者信息
- 1. 太原理工大学新型传感器与智能控制教育部重点实验室,山西 太原 030024
- 折叠
摘要
将一对可以产生荧光共振能量转移(FRET)的荧光分子分别作为供体染料和受体染料,以法布里-珀罗(F-P)微腔作为光学谐振腔,基于DNA分子的G-四链体结构研究FRET光微流激光的偏振特性.实验上,研究了5种不同K+浓度的DNA(其分子两端分别标记了一对可以产生FRET的荧光染料)溶液,用线偏振的泵浦光对其进行激发,将受体在平行偏振方向(平行于泵浦光偏振方向)的激光阈值曲线斜率和垂直偏振方向(垂直于泵浦光偏振方向)的激光阈值曲线的斜率的比值作为受体激光偏振度的检测信号.实验结果表明,随着DNA溶液中K+浓度的增加,受体激光的泵浦阈值逐渐降低,能量转换效率逐渐增加,而受体激光的斜率比逐渐变小,即偏振度逐渐降低.
Abstract
A pair of fluorescent molecules capable of producing fluorescence resonance energy transfer(FRET)were used as donor and acceptor dyes.The polarization properties of FRET optical microfluidic lasers were investigated based on the G-quadruplex structure of deoxyribonucleic acid(DNA)molecules using a Fabry-Perot(F-P)microcavity as an optical resonance cavity.In the experiment,five solutions of DNA of varying K+concentrations(whose molecular ends are each labeled with a pair of fluorescent dyes that can produce FRET)were studied and excited with linearly polarized pump light,and the ratio of the slope of the laser threshold curve of the acceptor in the parallel polarization direction(parallel to the pump light polarization direction)and the slope of the laser threshold curve(SER)in the vertical polarization direction(vertical to the pump light polarization direction)was used as the detection signal of the laser polarization of the acceptor.Findings indicate that as the K+content in the DNA solution increases,the pumping threshold of the acceptor laser decreases,energy conversion efficiency improves,and the slope ratio of the acceptor laser reduces,leading to decreased polarization.
关键词
荧光共振能量转移/光微流激光/法布里-珀罗微腔/激光偏振Key words
fluorescence resonance energy transfer/optofluidic laser/Fabry-Perot microcavity/laser polarization引用本文复制引用
出版年
2024
NETL
NSTL
万方数据