首页|基于FPGA的实时荧光相关光谱三元数字相关器的设计与实现

基于FPGA的实时荧光相关光谱三元数字相关器的设计与实现

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面向荧光相关光谱分析应用,研究基于现场可编程逻辑门阵列(FPGA)技术的多通道实时荧光相关光谱三元数字相关器的实现方法.硬件结构基于寄存器级硬件描述语言设计和实现.基于荧光相关光谱的特点,采用多采样时间相关和模块"分时复用"的设计思想,有效减少了运算量和硬件资源消耗.将二元相关的分时复用思想扩展至三元相关,提出了适用于三元相关的分时复用方法,实现了基于FPGA的实时计算的三元数字相关器.通过对称归一化的计算方法保证了相关函数的计算精度.光子相关器基于Xilinx Zynq-7100 FPGA芯片实现,实现了三路自相关、三路互相关和一路三元相关的实时计算.实验验证了相关计算的准确性,最高时间分辨率可达40 ns,动态范围为1.57×107.
Design and Implementation of Real-Time Fluorescence Triple Correlation Spectroscopy Correlator Based on FPGA
To meet the requirements of multichannel fluorescence correlation spectroscopy with a wide dynamic range,a digital correlator based on field programmable gate array(FPGA)technology was designed and implemented.The hardware structure was designed and implemented using a register-level hardware description language.Based on the characteristics of photon pulse counting signals,the multi-sampling-time correlation and time-division multiplexing methods were used to calculate the correlation function,which substantially improved computing efficiency and optimized hardware resource utilization.The time-division multiplexing of cross correlation was extended to triple correlation,and the corresponding method was extended to adapt triple correlation.Ultimately,a real-time computing triple correlator based on FPGA was realized.The calculation method of symmetric normalization ensured the accuracy of the correlation function.The designed photon correlator was implemented based on a single Xilinx Zynq-7100 FPGA chip,which performed various functions,including the real-time correlation of three channels auto-correlation,three channels cross-correlation,and one channel triple correlation,with the time resolution of 40 ns and dynamic range of 1.57×107.

fluorescence correlation spectroscopydigital correlatormulti-sampling-time correlation methodphoton pulse countingtime-division multiplexing

孙亚楠、蔡萍、王伟达、陆欣伟、董朝青、任吉存

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上海交通大学电子信息与电气工程学院仪器科学与工程系,上海 200240

上海交通大学化学化工学院,上海 200240

荧光相关光谱 数字相关器 多采样时间相关法 光子脉冲计数 分时复用

国家重大科研仪器研制项目

22027803

2024

激光与光电子学进展
中国科学院上海光学精密机械研究所

激光与光电子学进展

CSTPCD北大核心
影响因子:1.153
ISSN:1006-4125
年,卷(期):2024.61(9)
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