荧光偏振成像技术及其生物学应用
Biological Applications of Fluorescence Polarization Imaging
杨子仪 1李世晗 2刘芷如 2钟素艺 2李美琪 3席鹏2
作者信息
- 1. 北京大学生物医学工程系,北京 100871;北京大学力学与科学工程系,北京 100871
- 2. 北京大学生物医学工程系,北京 100871
- 3. 北京大学生物医学工程系,北京 100871;北京大学生命科学学院,北京 100871
- 折叠
摘要
荧光偏振成像通过测量样品中荧光分子的偏振状态,得到样品的取向结构特征,在生物学领域有着广泛的应用.偏振成像常与其他荧光显微技术结合,用来解析细胞结构的排列方向、生物大分子的实时动态取向和组织的有序性等信息,以研究细胞的生理过程、药物对细胞的作用及异常结构的检测.本文阐述了荧光偏振成像在生物学等领域的应用,对利用偏振信息获取不同样品取向结构的研究方法进行分析比较,并对其未来发展趋势进行展望.
Abstract
Fluorescence polarization imaging can obtain the orientation and structural features of the sample by measuring the polarization parameters of fluorescent molecules,owing to which it is widely used in biology research.Moreover,it is often combined with other fluorescence microscopy techniques to analyze the arrangement of cellular structures,the real-time dynamic orientation of biomolecules,and the organization of tissues.This approach enables research on cellular physiological processes,the effects of drugs on cells,and the detection of abnormal structures.In this study,we focus on the applications of fluorescence polarization imaging in biology research,including the analysis and comparison of different methods that utilize polarization information to obtain orientation structures of different samples.Furthermore,we provide insights into future development trends in this field.
关键词
荧光偏振成像/分子取向解析/生物显微成像Key words
fluorescence polarization imaging/orientation mapping/biological microscopic imaging引用本文复制引用
出版年
2024
NETL
NSTL
万方数据