pH响应型BSA-GMA 3D水凝胶结构的飞秒激光直写
Fabrication of pH-Responsive BSA-GMA 3D Hydrogel Structures via Femtosecond Laser Direct Writing
李腾 1武昕宜 1段琦 1宾凡淳 1牛梦瑶 1郑美玲2
作者信息
- 1. 中国科学院理化技术研究所有机纳米光子学实验室,北京 100190;中国科学院大学,北京 101407
- 2. 中国科学院理化技术研究所有机纳米光子学实验室,北京 100190
- 折叠
摘要
pH响应水凝胶由于其具有酸/碱依赖性导致的结构或体积变换能力,在智能操纵致动器、药物递送、组织工程等领域引起了广泛关注.随着生物医学和组织工程的发展,刺激响应生物材料微观结构的制备至关重要.牛血清白蛋白(BSA)因具有无毒、生物降解性、生物相容性等优点而被广泛应用于组织工程与药物递送等领域.本文重点开展了基于牛血清白蛋白-甲基丙烯酸缩水甘油酯(BSA-GMA)水凝胶的宏观pH响应、三维(3D)水凝胶微结构的飞秒激光直写与微观pH响应,以及细胞活性的相关研究.结果表明,飞秒激光直写可以制备出高精度的BSA-GMA 3D水凝胶微结构,并且BSA-GMA水凝胶的pH响应能力会随着BSA-GMA浓度的增大或甲基丙烯酸化度的减小而增强.相较于BSA水凝胶,BSA-GMA在光聚合过程中不消耗氨基酸基团,使得同等浓度下的BSA-GMA水凝胶含有更多的氨基与羧基,从而赋予BSA-GMA 3D水凝胶更强的pH响应性.软骨细胞在BSA-GMA支架上的共聚焦荧光图像和相对细胞增长率结果进一步表明,BSA-GMA水凝胶结构具有良好的生物相容性.这种具有可控形态和pH响应特性的蛋白质微结构在组织工程、生物医学和生物传感器领域具有潜在的应用前景.
Abstract
pH-responsive hydrogels have garnered considerable attention in actuator manipulation,drug delivery,and tissue engineering because of their capacity to undergo structural or volumetric changes in response to acid/base alterations.The fabrication of microstructures of stimuli-responsive biomaterials is crucial in the development of biomedicine and tissue engineering.Bovine serum albumin(BSA)is commonly used in tissue engineering and drug delivery because of its non-toxic,biodegradable,and biocompatible properties.This study presents the macroscopic pH response of BSA-glycidyl methacrylate(BSA-GMA)hydrogels,the microscopic pH response of three-dimensional(3D)hydrogel microstructures polymerized by femtosecond laser direct writing,and cell viability studies.Femtosecond laser direct writing enables the creation of high-precision 3D structures of BSA-GMA hydrogels.The results indicate that the pH responsiveness of the BSA-GMA hydrogels increased with either increasing concentration or decreasing methacrylation degree of BSA-GMA.Unlike the BSA hydrogel,the photopolymerization of the BSA-GMA hydrogel by femtosecond laser direct writing does not deplete amino acid groups.Consequently,the 3D BSA-GMA hydrogel demonstrates a stronger pH response because it contains more amino and carboxyl groups.Furthermore,confocal fluorescence imaging and analysis of relative cell growth rates of chondrocytes on the BSA-GMA scaffolds indicate that the BSA-GMA hydrogel has good biocompatibility.These protein microstructures with controlled morphology and pH-responsive properties have potential applications in tissue engineering,biomedicine,and biosensors.
关键词
牛血清白蛋白/飞秒激光直写/三维微结构/pH响应/生物相容性Key words
bovine serum albumin/femtosecond laser direct writing/three-dimensional microstructure/pH response/biocompatibility引用本文复制引用
出版年
2024
NETL
NSTL
万方数据