Study on seedless Shatian Pumello induced by Laser mutagenesis
From 1988 to 1992 about 16200 buds of Shantian Pumello had been radiated by laser.Then they had been grafted onto 320 Shantian Pumello trees.These tree's age were about 10 years old and they were distributed over three region of different longitude and latitude.The seedless or pauciseed Shatian Pumello fruit were been gained.The analysis of isoenzyme,the measuring of ratio of pollen abortion for L1 and L2 generation,the random amplified polymorphic DNA(RAPD) analysis for tender leaves of L3 generation,show that the mutant group compared with CK,have difference in isoenzyme activity,pollen abortion and RAPD.It demonstrated that the variation of Shatian Pumello induced by laser may be heritable.