Membrane Surface Charge Of Young and Old RBC Based On Percoll Density Gradient Separation Method
The surface electrical characteristics of aging erythrocytes are closely related to its structural func-tions. Owing to the uncertainty of the difference between young and old RBC’s membrane surface charge, in this study, we compared the separated results of young and old erythrocytes by the methods of high speed centrifugation and Percoll density gradient separation. With the results of membrane surface sialic acid content and membrane elas-tic modulus Kc, it was found that using Percoll density gradient separation method to separate young and old erythro-cytes is better than using high speed centrifugation, and the cell age gap between young and old erythrocytes is more greater. Further, four kinds of erythrocytes with different cell ages were separated by Percoll. Their surface membrane Zeta potential and sialic acid content are obviously different, indicating that the membrane surface negative charge of young RBC is different from the old one, which would get lower and lower with the increased cell age. The result is benefit to solve the mechanism of aging erythrocytes and the clearance pathways of aging erythrocytes in vivo.
Erythrocytecell agesurface chargePercoll density gradient separation method
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NSTL
万方数据
维普
