基于Design-Expert优化一款中药空气抑菌剂配方的研究
Optimization of the Formula of an Air Antibacterial Agent of Traditional Chinese Medicine Based on Design-Expert
黄晓珊 1王亚琴 1卢佳佳 1曾俊棠 1黄烁 1范宇梦 1吕立铭 1李大炜1
作者信息
- 1. 惠州卫生职业技术学院,广东惠州,516025
- 折叠
摘要
目的:以多种中药挥发油作为原料,开发一款高效广谱抑菌的空气抑菌剂.方法:选取丁香、艾叶、香茅 3 种挥发油,采用Design-Expert 12.0 中自带的Optimal(custom)试验设计进行最优混料配方筛选试验.通过采样我校饭堂空间空气浮游菌,先按软件设计配方加样抑菌孵育并计数,再拟合出孵育菌落数与各挥发油配方比例的模型方程,最后计算出最佳抑菌效能配方比例,并采用实验室试验和现场试验验证优选配方.结果:利用 286 组抑菌孵育数据计算得出,孵育菌落数(Y)与各挥发油配方比例之间的拟合方程为Y=17.60A+53.01B +36.76C-106.11AB-84.87AC-63.93BC+782.79A2BC-1178.89AB2C +413.15ABC2.丁香与艾叶、香茅挥发油抑菌的交互作用对孵育菌落数量有显著影响(p＜0.05).最终选择的丁香、艾叶、香茅挥发油比例为 45.2:41.6:13.2,该配方的实验室试验和现场试验验证了其抑菌效能良好.结论:中药抑菌挥发油复配后具有明显的增效作用.该配方的空气抑菌剂抑菌效果明显,气味芳香易于被人们接受,具有良好的市场前景和推广价值.
Abstract
Objective:To develop an effective and broad-spectrum air disinfectant using volatile oil of traditional Chinese medicine as raw materials.Methods:Three volatile oils,clove(Syzygium aromaticum),mugwort(Artemisia argyi),and lemongrass(Cymbopogon citratus),were selected.The D-optimal mixture design was used in Design-Expert 12.0 and a total of 16 runs were formulated.The planktonic bacteria in the canteen of our university was sampled.Added antibacterial agent and incubation,the number of colonies was counted.The formula designed by the software and fitting equation between the number of colonies and the proportion of each volatile oil formula was then fitted.Finally,the proportion of the optimal antibacterial efficacy formula was calculated by software,then verified by laboratory test and field test.Results:The fitting equation between the number of colonies(Y)and the proportion of each volatile oil formula was Y=17.60A+53.01B +36.76C-106.11AB-84.87AC-63.93BC+782.79A2BC-1178.89AB2C +413.15ABC2.The antibacterial interaction between clove and mugwort/lemongrass volatile oil had a significant effect on the number of colonies(p＜0.05).The final ratio of clove,mugwort,and lemongrass volatile oils was 45.2:41.6:13.2.The antibacterial efficacy of the formula was verified by laboratory test and field test.Conclusion:The antibacterial effect of volatile oil of traditional Chinese medicine can be significantly increased after compound.The formula of air antibacterial agent has obvious antibacterial effect,aromatic and easy to be accepted by people,and has good market prospects and promotion value.
关键词
中药挥发油/空气抑菌剂/最优混料设计/抑菌试验Key words
volatile oil of traditional Chinese medicine/air antibacterial agent/D-Optimal mixture design/antibacterial test引用本文复制引用
基金项目
2022年广东省科技创新战略专项"'攀登计划'专项(pdjh2022b1012)
广东省教育厅2021年度普通高校重点科研平台和项目(2021ZDZX2082)
出版年
2024
NETL
NSTL
维普
万方数据