HPLC法测定泡吴茱萸中柠檬苦素、吴茱萸碱和吴茱萸次碱含量

HPLC Determination of Limonin,Codiamine and Rutacarpine in Processed Evodia Rutaecarpa(Juss.)Benth

谭甜霞 ¹姜军华¹

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作者信息

1. 江西省药品检验检测研究院,国家药品监督管理局中成药质量评价重点实验室,江西省药品与医疗器械质量工程技术研究中心,江西南昌,330029
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摘要

目的:建立用高效液相色谱法(HPLC)测定泡吴茱萸中柠檬苦素、吴茱萸碱、吴茱萸次碱三种成分的含量.方法:色谱柱为Eclipse XDB C18(4.6 × 250mm,5μm),流动相:A乙腈,B甲醇,C 0.05％磷酸溶液,各流动相配比为30∶20∶50,流速:1.000mL·min-1,检测波长:215nm,柱温:30℃.结果:柠檬苦素、吴茱萸碱、吴茱萸次碱三者依次在 0.1303～1.6291 μg(r=0.9995)、0.0500～0.6251 μg(r=1.0000)、0.0354～0.4429μg(r=0.9999)范围内与其峰面积有良好的线性关系,且三者的平均加样回收率和RSD值分别为 100.11％(RSD=0.7％)、100.74％(RSD=1.4％)和 99.39％(RSD=1.3％).结论:该方法操作简单,结果稳定,有着高准确度,适用于泡吴茱萸中柠檬苦素、吴茱萸碱、吴茱萸次碱含量的测定.

Abstract

Objective:To establish a HPLC method for the determination of limonin;codiamine and rutacarpine in processed Evodia rutaecarpa(Juss.)Benth.Methods:The analysis was performed on Eclipse XDB C18(4.6 x 250mm,5 μm)column.The mobile phase was acetonitrile-methanol-0.05％phosphoric acid solution(30∶20∶50)at the flow rate of 1.000 mUmin.The detection wavelength was 215 nm and column temperature was set at 30 ℃.Results:The linear ranges of limonin;codiamine and rutacarpine were 0.1303～1.6291 μg(r=0.9995),0.0500～0.6251 μg(r=1.0000),0.0354～0.4429 μg(r=0.9999),respectively.The average recovery rates were 100.11％(RSD=0.7％),100.74％(RSD=1.4％),99.39％(RSD=1.3％).Conclusion:This method is simple,accurate and proper for simultaneous determination of limonin,codiamine and rutacarpine in processed Evodia rutaecarpa(Juss.)Benth.

关键词

HPLC/含量测定/柠檬苦素/吴茱萸碱/吴茱萸次碱/泡吴茱萸

Key words

HPLC/content determination/limonin/codiamine/rutacarpine/processed evodia rutaecarpa(Juss.)benth

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基金项目

江西省中医药标准化研究项目(2021A12)

出版年

2024

江西化工

江西化学化工学会江西省化学工业研究所

江西化工

影响因子：0.272

ISSN：1008-3103

参考文献量13

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