Synthesis and Characterization of a Fluorescent Probe for the Detection of Hydrogen Sulfide Based on the Turn-Off Structure of Xanthene
Hydrogen sulfide(H2S),as one of the living substances,participates in a variety of physiological processes in organisms,such as maintaining cell REDOX balance,ischemia-reperfusion injury,anti-inflammation and apoptosis.Therefore,in order to better understand the role of H2S in biological systems and accurate diagnosis of diseases,compound 1 with the structure of oxacanthrene was synthesized by Suzuki coupling reaction and substitution reaction,and its fluorescence response to H2S was studied in detail.Compound 1 itself emits bright orange fluorescence,which weakens or even disappears in response to hydrogen sulfide,and its response to other anions and amino acids is extremely weak.The validation mechanism has been verified by high-resolution mass spectrometry.Probe 1 recognized H2S with good selectivity and anti-interference ability and low detection limit.Most importantly,the probe has been successfully applied for fluorescence imaging of exogenous H2S in living cells.
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