Study on the Extraction Process of Flavonoids from Caesalpinia Minax and its Antioxidant Activity
The extraction conditions of the flavonoids were improved using the response surface approach based on a one-way experiment,and the antioxidant activity of the flavonoids was tested using the DPPH method and the hydroxyl radical hair method.The average flavonoid extraction rate was 1.43％when the volume fraction of ethanol was 61％,the microwave power was 614 W,the microwave period was 7 minutes,the liquid-to-feed ratio was 50∶1 mL/g,and the extraction temperature was 70 ℃.Caesalpinia minax's ability to scavenge DPPH and hydroxyl radicals was favorably correlated to its mass concentration,with the maximum scavenging rates of 83.89％and 82.07％,respectively.The IC50 values for Caesalpinia minax flavonoid were 17 μg/mL and 130μg/mL,demonstrating that it exhibits antioxidant action.
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