Research on the Law of Quantity Transfer of Atractylodis Rhizoma Standard Decoction
Objective:To study the quantitative transfer law of atractylodin and chlo-rogenic acid in Atractylodis rhizoma standard decoction,and to provide reference for establishing the quality standard of Atractylodis Rhizoma formula granules and its compound preparations.Methods:Taking atractylodin and chlo-rogenic acid as index components,combined with the extraction rate,fingerprint similarity,component transfer rate and index component content,the fingerprint of Atractylodis rhizoma pieces and standard decoction was established by HPLC,and the transfer law of value from Atractylodis rhizoma pieces to standard decoction was determined.The fingerprint of Atractylodis rhizoma standard decoction demarcates 8 common peaks,among which 5 common peaks were transmitted to the standard decoction,and 2 common components,namely atractylodin and chlo-rogenic acid,were identified.According to the peak area cluster analysis of standard decoction,which was consistent with the similarity of fingerprint.The results of content determination showed that the extraction rate of standard decoction ranged from 21.26％to 29.97％.The content of atractylodin ranged from 0.570％to 1.283％,and the transfer rate was 0.04％to 0.25％.The content of chlo-rogenic acid ranged from 0.033％to 0.223％,and the transfer rate was 31.09％to 67.41％.Conclusion:The results show that the transfer rate of chlo-rogenic acid in the standard decoction of Atractylodis rhizoma is high,but the transfer rate of atractylodin in Pharmacopoeia is extremely low,which indicates that chlo-rogenic acid can be used as one of the quality control components of the standard decoction of Atractylodis rhizoma,and atractyl as the index component of the quality control of the standard decoction needs further study and discussion.However,the HPLC method is stable and reliable,which can provide reference for the quality control of Atractylodis rhizoma standard decoction.
万方数据
维普
