摘要
目的:评价高效液相色谱法测定鸡蛋中四种氟喹诺酮药物残留的不确定度评定.方法:依据农业部781号公告-6-2006,测定鸡蛋中四种氟喹诺酮类兽药残留量.通过建立数学模型,分析系列标曲的配置、仪器设备的检定、试样称量、重复性实验以及样品溶液制备情况,得出鸡蛋中达氟沙星、恩诺沙星、沙拉沙星、环丙沙星四种氟喹诺酮兽药残留的不确定度.结果:鸡蛋中达氟沙星、恩诺沙星、沙拉沙星、环丙沙星四种氟喹诺酮兽药残留分别为 X环=85.009±0.03386 μg/kg(k=2),X恩=44.920±0.05950 μg/kg(k=2),X达=55.195±0.04622 μg/kg(k=2),X沙=96.312±0.03734 μg/kg(k=2).结论:结果的不确定度源于系列标曲的配置、仪器设备的检定、试样称量、重复性实验以及样品溶液制备等过程,建议提高实验人员的操作水平,从而确保检测结果的准确性.
Abstract
Objective:Uncertainty evaluation for the determination of four fluoroquinolone residues in egg by High Performance Liquid Chromatography.Method:According to The determinatian of quinolones residues in egg by HPLC method(Ministry of Agriculture Annough the establishment of a mathematical model,analysis and evaluation of a series of standard curve configuration,verification of instruments and equipment,sample weighing,repetitive experiments and sample solution preparation,the uncertainty of the residues of four fluoroquinone drugs,enrofloxacin,ciprofloxacin,danofloxacin and salafloxacin,is obtained.Result:Evaluation of The uncertianty of the determination of four fluoroquinolone residues in egg cadmium concent in rice were Xcipro=85.009±0.03386 μg/kg(k=2),Xenro=44.920±0.05950 μg/kg(k=2),Xpano=55.195±0.04622 μg/kg(k=2),Xsara=96.312±0.03734 μg/kg(k=2).Conclusion:The main sources of measurement uncertainty are a series of standard curve configuration,verification of instruments and equipment,sample weighing,repetitive experiments and sample solution preparation,which should be controlled during the actual detection process to ensure the accuracy of the measurement results.
维普
万方数据