首页|高效液相色谱法测定化妆品中马来酸氯苯那敏的研究

高效液相色谱法测定化妆品中马来酸氯苯那敏的研究

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本文建立了一种高效液相色谱法,用于检测化妆品中马来酸氯苯那敏(一种抗敏剂)的含量.其中,水剂类、凝胶类、膏霜类、乳液类、贴膜类、粉剂类等6类剂型的化妆品采用以甲醇为溶剂的50℃水浴超声提取;油剂类和蜡基类的化妆品使用正己烷为溶剂,在60℃水浴中超声溶解样品,使用乙腈进行提取,并取5mL乙腈溶液的下层,用氮气吹干,加入同体积甲醇复溶,完成溶剂置换.过滤膜后,经C18色谱柱分离,以磷酸二氢铵缓冲盐溶液和乙腈为流动相,检测波长为262nm,外标法定量.结果表明,所建立方法线性关系良好,检出限为15 μg/g,定量限为50μg/g,1倍、2倍、10倍定量限的加标回收率分别为85.3%~104.9%,相对标准偏差(RSD)为1.54%~9.42%(n=6).通过对33种化妆品进行检测,研究发现其中4种防晒化妆品中含有马来酸氯苯那敏.本方法快速、简便、灵敏度高适用于检测化妆品中马来酸氯苯那敏,前处理方法还可推广至化妆品中其他类亲水性待测目标物的提取.
Study on Determination of Chlorpheniramine Maleate in Cosmetics by High Performance Liquid Chromatography
A high performance liquid chromatography(HPLC)method was established for detecting the content of chlorpheniramine maleate(an antihistamine)in cosmetics.Methanol was used as the solvent for six types of cosmetics,including waters,gels,creams,emulsions,films,and powders.The samples were extracted by ultrasound in 50℃ water bath.For oil and wax-based cosmetics,n-hexane is used as the solvent,and the samples are ultrasonically dissolved in a 60℃ water bath.Then they were extracted with acetonitrile,and take the lower layer of 5 mL acetonitrile solution nitrogen blowing until dry,adding the same volume of methanol to redissolve,complete the solvent replacement,and then separated by C18 chromatographic column.The mobile phase were ammonium dihydrogen phosphate buffered solution and acetonitrile.The detection wavelength was 262 nm,and the external standard method was used for quantification.The results showed that the developed method had good linearity,the detection limit was 15 μg/g,and the quantification limit was 50 μg/g.The 1,2,and 10 times limit of quantification recoveries were 85.3%to 104.9%,and the RSDs were 1.54%to 9.42%(n=6).Chlorpheniramine maleate was detected in 4 types of sunscreen cosmetics by testing 33 types of cosmetics.This method is fast,simple,and highly sensitive,making it suitable for the detection of chlorpheniramine maleate in cosmetics.The pretreatment method can also be extended to the extraction of other hydrophilic target substances in cosmetics.

cosmeticssunscreenhigh performance liquid chromatographyantisensitizerschlorpheniramine maleate

马驰远、段吴燕、马欣欣、宋信卿、周蓉、吴森红、万建春、李仕祥

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南昌海关技术中心,江西南昌,330038

化妆品 防晒霜 高效液相色谱 抗敏剂 马来酸氯苯那敏

2024

江西化工
江西化学化工学会 江西省化学工业研究所

江西化工

影响因子:0.272
ISSN:1008-3103
年,卷(期):2024.40(6)