Study on Determination of Chlorpheniramine Maleate in Cosmetics by High Performance Liquid Chromatography
A high performance liquid chromatography(HPLC)method was established for detecting the content of chlorpheniramine maleate(an antihistamine)in cosmetics.Methanol was used as the solvent for six types of cosmetics,including waters,gels,creams,emulsions,films,and powders.The samples were extracted by ultrasound in 50℃ water bath.For oil and wax-based cosmetics,n-hexane is used as the solvent,and the samples are ultrasonically dissolved in a 60℃ water bath.Then they were extracted with acetonitrile,and take the lower layer of 5 mL acetonitrile solution nitrogen blowing until dry,adding the same volume of methanol to redissolve,complete the solvent replacement,and then separated by C18 chromatographic column.The mobile phase were ammonium dihydrogen phosphate buffered solution and acetonitrile.The detection wavelength was 262 nm,and the external standard method was used for quantification.The results showed that the developed method had good linearity,the detection limit was 15 μg/g,and the quantification limit was 50 μg/g.The 1,2,and 10 times limit of quantification recoveries were 85.3%to 104.9%,and the RSDs were 1.54%to 9.42%(n=6).Chlorpheniramine maleate was detected in 4 types of sunscreen cosmetics by testing 33 types of cosmetics.This method is fast,simple,and highly sensitive,making it suitable for the detection of chlorpheniramine maleate in cosmetics.The pretreatment method can also be extended to the extraction of other hydrophilic target substances in cosmetics.