Establishment of TLC Identification Method for Medicinal Herbs of Banfenghe
S.cathayensis and P.heterophyllum reference herbal solutions were used as reference substances and the effects of preparation method of test solution,developing agent,sample amount,spreading distance and color developer on TLC analysis,Saturationmode,inspection mode were investigated and the best TLC conditions of medicinal herbs of banfenghe were determined.Thin-layer chromatography GF254silica gel plate was used to prepare the test solution with 100％methanol as extraction solvent,Cyclohexane:ethyl acetate:formicacid=7:2:0.1 as developing agent,sprayed with 10％Ethanol sulfate solution,dried by hot air for color development,and examined under the 365nm UV lamp.The obtained medicinal herbs of Banfenghe layer chromatography has good separation and color development effect,clear spots and good characteristics.The method is safe,easy to operate and highly specific,and can be used as a TLC identification method for medicinal herbs of Banfenghe.
万方数据
维普
