Study on the rapid identified of COVID-19 cases based on nanoparticle targeting whole genome sequencing technology
Objective Exploring the method of rapid identified of COVID-19 for providing a technical support for New Coron-avirus's prevention and control.Methods The genome sequencing of novel coronavirus pneumonia diagnosed by overseas patients was carried out using New Coronavirus(SARS-CoV-2)based on MinION Mk1C platform and Ion Torrent S5 platform.The artic-ncov2019 software,CLC genomics workbench(version 21.0)software and DNAStar software were used for data processing and analysis.Results Based on minion mk1c platform and ion torrent S5 platform,the whole genome data of sars-cov-2 were 29848 BP and 29801 BP in 7 hours and 30 hours respectively.The shared 29801 BP data shared 100％homology,and they were identi-fied as new coronavirus b1.1.7 variant.Conclusion The whole genome sequencing technology based on minion mk1c platform can shorten the detection cycle to about 7 hours,which can realize rapid and accurate real-time sequencing of sars-cov-2.
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