Study on the in vitro antibacterial effect of liposomal linolenic acid(LipoLLA)on Helicobacter pylori
Objective The purpose of this study is to explore the antibacterial effects of liposomal linolenic acid(LipoLLA)on Helicobacter pylori and provide theoretical basis for the clinical application of LipoLLA in the treatment of Helicobacter pylori in-fection.Methods In this study,50 strains of Helicobacter pylori were collected,and the minimum inhibitory concentration(MIC)of LipoLLA and four antibiotics(AMX,MTZ,LVFX,and CAM)used alone or in combination against Helicobacter pylori was de-termined by broth microdilution method.The antibacterial activity of LipoLLA at different concentrations,times,and pH values was evaluated by inhibition curve.The levels of AST,GLU,and ATP in the supernatant of the medium after LipoLLA treatment were measured to explore its antibacterial mechanism.Results The MIC range of LipoLLA was 3.75～15 μg/mL,and when used in combination with the four antibiotics(AMX,MTZ,LVFX,and CAM),it showed a synergistic antibacterial effect.The antibacterial effect of LipoLLA was influenced by concentration,time,and pH value.Furthermore,the outer membrane barrier of Helicobacter pylori was disrupted after LipoLLA treatment,leading to an increase in the levels of AST,GLU,and ATP in the medium.Conclu-sion LipoLLA can effectively kill bacteria by disrupting the outer membrane barrier of Helicobacter pylori,and it can be used in combination with traditional antibiotics to enhance the antibacterial effect against Helicobacter pylori.
万方数据
维普
