The effects of Cystatin SN on zebrafish xenograft model of human esophageal squamous cell carcinoma
Objective To explore the effects of cystatin SN(CST1)expression on the tumor migration and angiogenesis by estab-lishing the human esophageal squamous cell carcinoma(ESCC)zebrafish xenograft model.Methods ESCC cells were stably knocked down and overexpressed of CST1 by constructed CST1 overexpression and knockdown lentivirus transfection,respectively,and then cell migration and invasion ability were detected by Transwell assay.Subsequently,overexpression and knockdown human ESCC cells were injected into the yolk sac of zebrafish embryos to establish ESCC zebrafish xenograft model,respectively,and then the tumor migration and angiogenesis of sub intestinal veins(SIVs)of zebrafish model were observed.Results The results in vitro experi-ments showed that CST1 overexpression significantly enhanced ECC cell migration and invasion,whereas CST1 knockdown sig-nificantly inhibited ESCC cell migration and invasion.The survival rate of zebrafish xenograft model of ESCC was more than 0.8 when 300～500 ESCC cells were transplanted into the yolk sac.Compared to the negative control(NC)group,more tumor distinct migra-tion and hyperplasia of blood vessels of the SIVs in the CST1 overexpression group,whereas a reverse changes were observed in the CST1 knockdown group.Conclusions ESCC zebrafish xenograft model can be successfully established by injecting human esophageal squamous cell to the yolk sac of zebrafish,and then applied in vivo studies.CST1 expression might present a potential carcinogenic role on the development of ESCC by promoting the tumor migration and angiogenesis.
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维普
