Objective To investigate the effect of circular RNA(circRNA)circHIPK3 on clozapine induced injury of H9C2 my-ocardial cells and its related mechanisms.Methods CircHIPK3 was overexpressed or silenced in H9C2 cells by cell transfection and H9C2 cells were treated with clozapine.Real-time quantitative PCR(qRT-PCR)was used to detect the expression of circHIPK3.Methyl thiazolyl tetrazolium assay(MTT)was used to detect cell survival rate.Relevant kits were used to detect the levels of lactate de-hydrogenase(LDH),creatine kinase isoenzyme(CK-MB),Cardiac troponin(cTnI),Malondiadehyde(MDA),superoxide dismutase(SOD)and Glutathione peroxidase(GSH-Px).Flow cytometry was used to detect cell apoptosis rate and mitochondrial membrane poten-tial.Western blot was used to detect the protein expression of B-lymphocytoma-2-associated X protein(Bax),B-lymphocytoma-2(Bcl-2)and cleaved aspartate-specific cysteine proteinase-3(cleaved Caspase-3).Results Clozapine significantly increased circHIPK3,LDH,CK-MB,cTnI,MDA,apoptosis rate,Bax,cleaved Caspase-3 levels of H9C2 cells(P＜0.05),and significantly decreased cell survi-val rate,SOD,GSH-Px,Bcl-2 levels and mitochondrial membrane potential of H9C2 cells(P＜0.05).CircHIPK3 overexpression promot-ed the effect of clozapine on the above indexes(P＜0.05),while silencing circHIPK3 reversed the effect of clozapine on the above indexes(P＜0.05).Conclusion Clozapine can induce H9C2 myocardial cells injury by promoting circHIPK3 expression,and the mecha-nism may be related to the activation of oxidative stress and mitochondrial apoptosis pathways by circHIPK3.
维普
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