miR-93-5p/FOXJ2轴介导胃癌细胞的生物学功能
miR-93-5p/FOXJ2 axis mediates biological function of gastric cancer cells
沈蕾 1钮渊杰 2宋巍 2陈二林3
作者信息
- 1. 南通大学附属医院 医学检验科,江苏 226001
- 2. 南通大学医学院
- 3. 南通大学附属医院 普通外科,江苏 226001
- 折叠
摘要
目的:评估miR-93-5p对胃癌细胞的调节和功能.方法:采用qPCR检测miR-93-5p在胃癌组织、胃癌细胞系中表达,MTT、克隆形成、Transwell实验检测miR-93-5p对胃癌细胞增殖、侵袭的影响,双荧光素酶报告基因实验检测miR-93-5p与FOXJ2 的靶向关系.结果:miR-93-5p在胃癌细胞系和胃癌组织中表达升高,抑制miR-93-5p表达后胃癌细胞增殖和侵袭能力受抑.双荧光素酶报告基因检测显示,miR-93-5p mimic显著降低MKN28 和MKN45细胞Wt-FOXJ2 相对荧光素酶活性,FOXJ2 为胃癌细胞中miR-93-5p的直接靶基因.结论:miR-93-5p靶向FOXJ2促进胃癌细胞的增殖和侵袭,miR-93-5p/FOXJ2 轴可能是判断胃癌预后的生物标志物和治疗靶点.
Abstract
Objective:To evaluate the regulation and function of miR-93-5p on gastric cancer cells.Methods:qPCR was used to detect the expression of miR-93-5p in gastric cancer tissue and gastric cancer cell lines.MTT,clone formation and Transwell were used to test the effect of miR-93-5p on proliferation and invasion of gastric cancer cells.Double luciferase reporter gene experiment was performed to test the targeting relationship between miR-93-5p and FOXJ2.Results:The expression of miR-93-5p was upregulated in gastric cancer cell lines and tissues,and the prolifera-tion and invasion ability of gastric cancer cells was inhibited after inhibiting miR-93-5p expression.Double luciferase re-porter gene detection showed that miR-93-5p mimic obviously decreased the relative luciferase activity of Wt-FOXJ2 in MKN28 and MKN45 cells,and FOXJ2 was the direct target gene of miR-93-5p.Conclusion:miR-93-5p targets FOXJ2 to promote the proliferation and invasion of gastric cancer cells,and miR-93-5p/FOXJ2 axis may be a biomarker and ther-apeutic target for the prognosis of gastric cancer.
关键词
胃癌/miR-93-5p/肿瘤标志物/增殖和侵袭/生物学功能Key words
gastric cancer/miR-93-5p/tumor markers/proliferation and invasion/biological function引用本文复制引用
基金项目
南通市科技计划指导性项目(JC22022010)
出版年
2024
NETL
NSTL
万方数据