miR-29c-3p调控ERLIN2抑制肺腺癌发展的作用
Role of miR-29c-3p in regulating ERLIN2 to suppress the development of lung adenocarcinoma
卞婷婷 1姜岱山 2张雅莉 1王斯楚 1刘益飞1
作者信息
- 1. 南通大学附属医院 病理科,江苏 226001
- 2. 南通大学附属医院 急诊医学科,江苏 226001
- 折叠
摘要
目的:探讨miR-29c-3p在肺腺癌(lung adenocarcinoma,LUAD)发生发展中的作用.方法:利用公共数据库分析miR-29c-3p和ERLIN2 在肺腺癌中的表达及其与临床病理参数的相关性.采用qRT-PCR检测miR-29c-3p在LUAD细胞系和正常支气管上皮细胞系中的表达,CCK8、细胞克隆形成、Transwell和伤口愈合实验评估miR-29c-3p在细胞增殖、迁移和侵袭中的作用,利用生物信息学工具预测miR-29c-3p下游靶基因,并通过双荧光素酶报告基因分析验证靶向关系.结果:miR-29c-3p在肺腺癌组织和细胞中低表达.miR-29c-3p低表达患者的预后较差,miR-29c-3p是肺腺癌患者的独立预后因素,并与淋巴结转移状态和临床分期密切相关.细胞实验表明,抑制miR-29c-3p可促进A549 细胞的增殖、迁移和侵袭.生物信息学分析表明,ERLIN2是miR-29c-3p的靶基因,二者表达呈负相关.通过双荧光素酶报告基因测定验证了上述靶向关系.在UALCAN数据库中,ERLIN2在肺腺癌组织中高表达,并与患者年龄、性别、吸烟、肿瘤分期、淋巴结转移和TP53突变状态密切相关,ERLIN2 高表达患者总生存率低于低表达患者.结论:miR-29c-3p通过靶向ERLIN2 抑制肺腺癌细胞的增殖、迁移和侵袭,是肺腺癌患者的独立预后因素.
Abstract
Objective:To explore the impact of miR-29c-3p in the development of lung adenocarcinoma.Methods:Public database was used to analyze the expression of miR-29c-3p and ERLIN2 in lung adenocarcinoma and its correla-tion with clinicopathological parameters.qRT-PCR was used to detect the expression of miR-29c-3p in LUAD cell line and normal bronchial epithelial cell line.The function of miR-29c-3p in cell proliferation,migration,and invasion was evaluated by CCK8,colony formation,Transwell assay and wound healing essay.Bioinformatics predicted the downstream target genes of miR-29c-3p,and verified the targeting relationship by Dual luciferase reporter assay.Results:miR-29c-3p was low expressed in lung adenocarcinoma tissues and cells.miR-29c-3p was an independent prognostic factor for lung adenocarcinoma and was closely related to lymph node metastasis status and clinical staging.Celluar experiments demon-strated that miR-29c-3p inhibitor promoted the proliferation,migration,and invasion of A549 cells.Bioinformatics analysis showed that ERLIN2 was a target gene for miR-29c-3p,and there was a negative correlation between their expression.The above targeting relationship was verified through dual luciferase reporter assay.In the UALCAN database,ERLIN2 is high-ly expressed in tumor tissue and is closely related to patient age,gender,smoking habits,tumor staging,lymph node metas-tasis status,and TP53 mutation status.Meanwhile,the higher the expression of ERLIN2,the shorter the overall survival time of patients.Conclusions:miR-29c-3p inhibits the proliferation,migration,and invasion of lung adenocarcinoma by target-ing ERLIN2,it is an independent prognosis factor.
关键词
肺腺癌/miR-29c-3p/ERLIN2/增殖/侵袭/迁移Key words
lung adenocarcinoma/miR-29c-3p/endoplasmic reticulum lipid raft-associated protein 2/prolifera-tion/migration/invasion引用本文复制引用
基金项目
南通市科技计划(MSZ19164)
南通市科技计划(JCZ20001)
南通市科技计划(JC12022016)
新锐肿瘤支持科研专项(cphcf-2022-206)
出版年
2024
NETL
NSTL
万方数据