Objective:To investigate the expression of globin transcription factor 1(GATA 1)and B-cell leukemi-a/lymphoma xL(BCL-xL)in peripheral blood cells of patients with myelodysplastic syndromes(MDS)and explore the mechanism related to Notch signaling pathway.Methods:A total of 36 MDS patients were selected as the MDS group and 20 healthy people who donated blood regularly were selected as the control group.Real-time fluorescence quantitative PCR was used to detect the expression of GATA 1,BCL-xL,neurogenic gene Notch homologous protein 1(Notch 1)and its downstream molecules HES 1 and Herp 2 in peripheral blood cells.The correlation between the expression of GATA 1,BCL-xL and platelet count,hemoglobin in MDS patients was analyzed.The apoptosis of peripheral blood cells was ob-served by flow cytometry.Results:Compared with the control group,the mRNA levels of GATA 1 and BCL-xL were sig-nificantly decreased in peripheral blood of MDS patients(P＜0.05),while the mRNA levels of Notch 1,HES 1 and Herp 2 were significantly increased(P＜0.05).Moreover,the expression of GATA 1 mRNA was positively correlated with the platelet count in MDS patients(r=0.7575,P=0.0181).Compared with the control group,the apoptotic cells increased in the MDS group(P＜0.01).Conclusion:The expression of GATA 1 and BCL-xL decreased,and the expression of Notch 1,HES 1 and Herp 2 increased in MDS patients,which may be the cause of significant increase in cell apoptosis.
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