Construction of a Prognostic Model for Lung Adenocarcinoma Based on Hypoxia-and Ferroptosis-Related Genes
Objective:To explore the prognostic value of hypoxia-and ferroptosis-related genes in lung adenocarci-noma(LUAD)and construct a nomogram model for predicting the prognosis of patients with LUAD.Methods:Hypoxia-and ferroptosis-related differentially expressed genes in lung adenocarcinoma were identified from the cancer genome atlas(TC-GA)database.A gene risk scoring system was constructed using LASSO-Cox regression,followed by the construction of a prognostic model for lung adenocarcinoma.The predictive effect of the model was evaluated through calibration curve,re-ceiver operating characteristic(ROC)curve,and decision curve analysis.Quantitative reverse transcription PCR(RT-qPCR)was used to verify the differential expression of risk genes between LUAD tissues and the adjacent normal tissues.Results:An eight-gene risk scoring system and a nomogram model for predicting the prognosis of LUAD patients were constructed,demonstrating good predictive effect.The expression of decorin(DCN)and platelet derived growth factor subunit B(PDGFB)decreased obviously,and the expression of dipeptidyl peptidase 4(DPP4),macrophage migration inhibitory factor(MIF),glyceraldehyde-3-phosphate dehydrogenase(GAPDH),lactate dehydrogenase A(LDHA),ribonucleotide reductase regulatory subunit M2(RRM2),and solute carrier family 2 member 1(SLC2A1)increased significantly(P<0.05).Conclu-sion:This nomogram model for predicting the prognosis of lung adenocarcinoma patients constructed in this study can pro-vide reference for personalized treatment.
lung adenocarcinomahypoxiaferroptosisprognostic model
万方数据
维普
