摘要
目的:研究吡拉西坦预处理对发生再灌注损伤心肌(MIRI)的作用机制.方法:40只SD大鼠按随机数字表法分为5组:假手术组、模型组、吡拉西坦低、中、高剂量组;除外假手术组,余组建立缺血再灌注的损伤模型.通过ELISA法测定血清中CK-MB、LDH、SOD、MDA、IL-6和TNF-α含量.测定心肌梗死部位面积的百分比以及心肌细胞凋亡率.结果:与模型组比较,吡拉西坦各组可明显改善再灌注后心肌损伤,降低心肌梗死面积.吡拉西坦各剂量组血清LDH、MDA、IL-6、TNF-α水平明显下降(P ＜0.05),SOD水
Abstract
Objective To study the mechanism of piracetam preconditioning on myocardial reperfusion injury (MIRI). Methods 40 SD rats were randomly divided into 5 groups: sham operation group, model group, low, medium and high dose piracetam groups; Except the sham operation group, the other groups established ischemia reperfusion injury models. Determination of CK-MB, LDH, SOD, MDA, IL-6 and TNF in serum by ELISA- α Content. The percentage of myocardial infarction area and the apoptosis rate of myocardial cells were measured. Results Compared with the model group, piracetam could significantly improve the myocardial injury after reperfusion and reduce the myocardial infarction area. Serum LDH, MDA, IL-6, TNF in each dose group of piracetam- α The level of SOD decreased significantly (P＜0.05), the level of SOD increased significantly (P＜0.05), and the level of CK-MB in middle and high dose groups decreased significantly (P＜0.01). Conclusion Piracetam can protect myocardium from reperfusion by inhibiting the level of oxidative stress after reperfusion, reducing the content of inflammatory factors and showing a certain dose correlation.
万方数据