摘要
目的:研究阴道微生物芯片技术在阴道分泌物念珠菌检测中的应用价值及对念珠菌检出率的影响.方法:2020年5月-2021年9月接收的183例疑似为念珠菌性阴道炎患者,采集阴道分泌物湿片镜检、阴道微生物芯片技术法、真菌显色培养,对比结果.结果:经"金标准"显示白色念珠菌占59.50％、光滑念珠菌占20.66％、热带念珠菌占12.40％、克柔念珠菌占8.26％;阴道微生物芯片技术对念珠菌类型检出率(67.21％)高于常规湿片镜检(56.28％)(P＜0.05).阴道微生物芯片技术对阴道分泌物念珠菌的诊断符合率为9
Abstract
Objective To study the application value of vaginal microarray technology in the detection of candida in vaginal secretions and its influence on the detection rate of Candida. Methods From May 2020 to September 2021, 183 patients with suspected candidal vaginitis were collected for vaginal secretion wet film microscopic examination, vaginal microbial chip technology and fungal chromogenic culture, and the results were compared. Results According to the "gold standard", Candida albicans accounted for 59.50％, Candida smooth accounted for 20.66％, Candida tropicalis accounted for 12.40％, and Candida krroux accounted for 8.26％; The detection rate of candida types by vaginal microbial chip technology (67.21％) was higher than that by conventional wet film microscopy (56.28％) (P＜0.05). The coincidence rate, sensitivity, specificity, positive predictive value and negative predictive value of vaginal microbial chip technology in the diagnosis of candida vaginalis were 95.63％, 97.52％,91.94％, 95.93％ and 95.00％, respectively, which were higher than 75.96％, 74.38％, 79.03％, 87.38％ and 61.25％ of the routine wet film microscopy (P＜0.05).Conclusion The detection of candida in vaginal secretions using vaginal microarray technology can effectively determine the type of Candida, and the diagnostic sensitivity, specificity and coincidence rate are high.
万方数据