Objective: To establish HPLC fingerprint method for the quality evaluation and discrimination of Callicaipa nudiflora. Methods:The chromatographic fingerprint of 16 samples was determined by injecting 10 μl of the sample solution each time om a reversed phase Thermo Acclaim TM 120 C18 column with the gradient elution solvent system composed of 0.1％ Formic acid solution and acetonitrile. The flow rate was 1.0 m1/min, the column temperature was 35 ℃ and the detection wavelength was set at 332nm.Results: The HPLC fingerprint of Callicarpa nudiflora was set up, which included 11 common peaks, and the similarities of 16 samples were well. Conclusion: The method which can be used for discrimination and the quality control of Callicarpa nudiflora.
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